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Drain off the fixative and abode the roots in 1% acetocarmine for 1 to 3 h. Calefaction until the acetocarmine begins to boil. Cut off the basis cap with a razor brand and clasp the meristematic tissue out with a lancet needle.

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D. Nicomrat and J. Anantasaran, "A Reliable Homemade Tissue Culture Protocol for Dendrobium Orchid Cultivation", Applied Mechanics and Materials, Vol. 804, pp. 227-230, 2015 Online since: October 2015

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The large scale commercial propagation of plant material based on plant tissue culture was pioneered in the USA. During the last thirty years, tissue culture-based plant propagation has emerged as one of the leading global agro-technologies. Between 1986 and 1993, the worldwide production of tissue cultured plants increased 50%.